Molecular Cloning and Expression Profiles of Thermosensitive TRP Genes in Agasicles hygrophila.

ABSTRACT: Global warming has gradually reduced the control efficacy of Agasicles hygrophila against the invasive weed Alternanthera philoxeroides. To better understand the summer collapse of A. hygrophila populations, we cloned the cDNA sequences of the high temperature-sensing TRPA1, Painless, and Pyrexia from A. hygrophila, and analyzed their temporal expressions and the impacts of high temperatures on their expression in eggs, the most vulnerable stage of A. hygrophila to hot temperatures. All the three genes obtained had the signature domains of TRPA channels and were constitutively expressed in eggs, larvae (L1, L2, L3), pupae, and adults, but AhPainless had the highest expression, followed by AhPyrexia, and AhTRPA1. T

SUBMITTER: Jia D 

PROVIDER: S-EPMC7570112 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature