Catalytic Gold Deposition for Ultrasensitive Optical Immunosensing of Prostate Specific Antigen.

ABSTRACT: A major challenge in the development of bioanalytical methods is to achieve a rapid and robust quantification of disease biomarkers present at very low concentration levels in complex biological samples. An immunoassay platform is presented herein for ultrasensitive and fast detection of the prostate-specific antigen (PSA), a well-recognized cancer biomarker. A sandwich type immunosensor has been developed employing a detection antibody labeled with inorganic nanoparticles acting as tags for further indirect quantification of the analyte. The required high sensitivity is then achieved through a controlled gold deposition on the nanoparticle surface, carried out after completing the recognition step of the immunoassay, thus effectively amplifying the size of the nanoparticles from nm to µm

SUBMITTER: Cid-Barrio L 

PROVIDER: S-EPMC7571086 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature