Hyperglucosuria induced by dapagliflozin augments bacterial colonization in the murine urinary tract.

ABSTRACT:

Aim

To test the effects of dapagliflozin-induced hyperglucosuria on ascending bacterial urinary tract infection (UTI) in a mouse model.

Methods

Dapagliflozin or canagliflozin was used to induce hyperglucosuria in non-diabetic adult female mice prior to transurethral inoculation with uropathogenic Escherichia coli (UPEC) or Klebsiella pneumoniae. Glucose, bacterial load, cytokines, neutrophil mobilization and inflammation during acute and chronic UTI were determined.

Results

Significant increase in UPEC load was observed in the urinary tract of hyperglucosuric mice compared with controls. Dapagliflozin-treated mice developed bacteraemia resulting in UPEC colonization of the spleen and liver at a higher frequency than controls. Chronic UTI in hyperglucosuric mice result

SUBMITTER: Saenkham P 

PROVIDER: S-EPMC7571118 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature