The FTO/miR-181b-3p/ARL5B signaling pathway regulates cell migration and invasion in breast cancer.

ABSTRACT:

Background

N6-methyladenosine (m⁶ A) RNA modification has been demonstrated to be a significant regulatory process in the progression of various tumors, including breast cancer. Fat mass and obesity-associated (FTO) enzyme, initially known as the obesity-related protein, is the first identified m⁶ A demethylase. However, the relationship between FTO and breast cancer remains controversial. In this study, we aimed to elucidate the role and clinical significance of FTO in breast cancer and to explore the underlying mechanism.

Methods

We first investigated the expression of FTO in breast cancer cell lines and tissues by quantitative reverse transcription-PCR (qRT-PCR), Western blotting, and immunohistochemistry. Wound healing assay and Transwell assay were

SUBMITTER: Xu Y 

PROVIDER: S-EPMC7571404 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature