PET imaging of distinct brain uptake of a nanobody and similarly-sized PAMAM dendrimers after intra-arterial administration.

ABSTRACT:

Introduction

We have recently shown that intracerebral delivery of an anti-VEGF monoclonal antibody bevacizumab using an intra-arterial (IA) infusion is more effective than intravenous administration. While antibodies are quickly emerging as therapeutics, their disadvantages such as large size, production logistics and immunogenicity motivate search for alternatives. Thus we have studied brain uptake of nanobodies and polyamidoamine (PAMAM) dendrimers.

Methods

Nanobodies were conjugated with deferoxamine (DFO) to generate NB(DFO)₂. Generation-4 PAMAM dendrimers were conjugated with DFO, and subsequently primary amines were capped with butane-1,2-diol functionalities to generate G4(DFO)₃(Bdiol)₁₁₀. Resulting conjugates were radiolabeled with z

SUBMITTER: Lesniak WG 

PROVIDER: S-EPMC7571511 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature