In situ structure of virus capsids within cell nuclei by correlative light and cryo-electron tomography.

ABSTRACT: Cryo electron microscopy (cryo-EM), a key method for structure determination involves imaging purified material embedded in vitreous ice. Images are then computationally processed to obtain three-dimensional structures approaching atomic resolution. There is increasing interest in extending structural studies by cryo-EM into the cell, where biological structures and processes may be imaged in context. The limited penetrating power of electrons prevents imaging of thick specimens (> 500 nm) however. Cryo-sectioning methods employed to overcome this are technically challenging, subject to artefacts or involve specialised and costly equipment. Here we describe the first structure of herpesvirus capsids determined by sub-tomogram averaging from nuclei of eukaryotic cells, achieved by cryo-elec

SUBMITTER: Vijayakrishnan S 

PROVIDER: S-EPMC7572381 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature