Hepatic stellate cell autophagy inhibits extracellular vesicle release to attenuate liver fibrosis.

ABSTRACT:

Background & aims

Autophagy plays a crucial role in hepatic homeostasis and its deregulation has been associated with chronic liver disease. However, the effect of autophagy on the release of fibrogenic extracellular vesicles (EVs) by platelet-derived growth factor (PDGF)-stimulated hepatic stellate cells (HSCs) remains unknown. Herein, we aimed to elucidate the role of autophagy, specifically relating to fibrogenic EV release, in fibrosis.

Methods

In vitro experiments were conducted in primary human and murine HSCs as well as LX2 cells. Small EVs were purified by differential ultracentrifugation. Carbon tetrachloride (CCl₄) or bile duct ligation (BDL) were used to induce fibrosis in our mouse model. Liver lysates from patients with cirrhosis or healthy controls w

SUBMITTER: Gao J 

PROVIDER: S-EPMC7572579 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature