Associations of smoking and air pollution with peripheral blood RNA N⁶-methyladenosine in the Beijing truck driver air pollution study.

ABSTRACT:

Background

Post-transcriptional modifications of RNA constitute fundamental mechanisms of gene regulation. N⁶-methyladenosine (m⁶A) is critical for health and disease and is modulated by cellular stressors. However, associations between environmental exposures and m⁶A have not been studied in humans. We aimed to examine associations between tobacco smoking and particulate air pollution with m⁶A and mRNA expression levels of its reader, writer and eraser (RWE) genes in blood.

Methods

Using the Beijing Truck Driver Air Pollution Study, we investigated global m⁶A in RNA from peripheral blood collected from 106 human subjects in Beijing, China, in 2008. We measured m⁶A with nano-flow liquid chromatography-tandem m

SUBMITTER: Kupsco A 

PROVIDER: S-EPMC7572654 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature