Cancer Immunotherapy via Targeting Cancer Stem Cells Using Vaccine Nanodiscs.

ABSTRACT: Cancer stem cells (CSCs) proliferate extensively and drive tumor metastasis and recurrence. CSCs have been identified in over 20 cancer types to date, but it remains unknown how to target and eliminate CSCs in vivo. Aldehyde dehydrogenase (ALDH) is a marker that has been used extensively for isolating CSCs. Here we present a novel approach to target and reduce the frequency of ALDH^high CSCs by vaccination against ALDH. We have identified ALDH1-A1 and ALDH1-A3 epitopes from CSCs and developed synthetic high-density lipoprotein nanodiscs for vaccination against ALDH^high CSCs. Nanodiscs increased antigen trafficking to lymph nodes and generated robust ALDH-specific T cell responses. Nanodisc vaccination against ALDH^high CSCs combined with anti-PD-L1 therapy ex

SUBMITTER: Hassani Najafabadi A 

PROVIDER: S-EPMC7572838 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature