Electrokinetic stacking of particle zones in confined channels enabling their UV absorbance detection on microchips.

ABSTRACT: In this article, we report a simple approach to stacking micro- and nanoparticle zones by electrokinetically migrating them through moderately confined channels of uniform cross-section. Experiments show the reported pre-concentration process to initiate at the tail end of the zone following its electrokinetic injection, with the stacked region migrating faster than the rest of the sample band. This effect causes the particles traveling in front to merge into the stacked region making it grow both in size and concentration. Because the stacked zone also gradually loses particles from its trailing edge, it eventually disintegrates upon running out of particles at its front end. Nevertheless, enhancements in peak height by over 100-fold were recorded using the reported approach for polystyre

SUBMITTER: Xia L 

PROVIDER: S-EPMC7573205 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature