Atypical TRAV1-2^- T cell receptor recognition of the antigen-presenting molecule MR1.

ABSTRACT: MR1 presents vitamin B-related metabolites to mucosal associated invariant T (MAIT) cells, which are characterized, in part, by the TRAV1-2⁺ αβ T cell receptor (TCR). In addition, a more diverse TRAV1-2^- MR1-restricted T cell repertoire exists that can possess altered specificity for MR1 antigens. However, the molecular basis of how such TRAV1-2^- TCRs interact with MR1-antigen complexes remains unclear. Here, we describe how a TRAV12-2⁺ TCR (termed D462-E4) recognizes an MR1-antigen complex. We report the crystal structures of the unliganded D462-E4 TCR and its complex with MR1 presenting the riboflavin-based antigen 5-OP-RU. Here, the TRBV29-1 β-chain of the D462-E4 TCR binds over the F'-pocket of MR1, whereby the complementarity-determining reg

SUBMITTER: Awad W 

PROVIDER: S-EPMC7573270 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature