The Twin-Arginine Translocation System Is Important for Stress Resistance and Virulence of Brucella melitensis.

ABSTRACT: Brucella, the causative agent of brucellosis, is a stealthy intracellular pathogen that is highly pathogenic to a range of mammals, including humans. The twin-arginine translocation (Tat) pathway transports folded proteins across the cytoplasmic membrane and has been implicated in virulence in many bacterial pathogens. However, the roles of the Tat system and related substrates in Brucella remain unclear. We report here that disruption of Tat increases the sensitivity of Brucella melitensis M28 to the membrane stressor sodium dodecyl sulfate (SDS), indicating cell envelope defects, as well as to EDTA. In addition, mutating Tat renders M28 bacteria more sensitive to oxidative stress caused by H₂O₂ Further, loss of Tat significantly attenuates B.

SUBMITTER: Yan X 

PROVIDER: S-EPMC7573438 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature