Evaluation of the Immunogenicity and Protective Efficacy of an Enterotoxigenic Escherichia coli CFA/I Adhesin-Heat-Labile Toxin Chimera.

ABSTRACT: Recent efforts to develop an enterotoxigenic Escherichia coli (ETEC) vaccine have focused on the antigenically conserved tip adhesins of colonization factors. We showed previously that intranasal immunization with dsc₁₉CfaE, a soluble variant of the in cis donor strand-complemented tip adhesin of a colonization factor of the class 5 family (CFA/I) fimbria, is highly immunogenic and protects against oral challenge with CFA/I-positive (CFA/I⁺) ETEC strain H10407 in the Aotus nancymaae nonhuman primate. We also reported a cholera toxin (CT)-like chimera (called dsc₁₉CfaE-CTA2/CTB) in which the CTA1 domain of CT was replaced by dsc₁₉CfaE that was strongly immunogenic when administered intranasally or orogastrically in mice. Here,

SUBMITTER: O'Dowd A 

PROVIDER: S-EPMC7573451 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature