Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation.

ABSTRACT: Different types of carbon materials are biocompatible with neural cells and can promote maturation. The mechanism of this effect is not clear. Here we have tested the capacity of a carbon material composed of amorphous sp³ carbon backbone, embedded with a percolating network of sp² carbon domains to sustain neuronal cultures. We found that cortical neurons survive and develop faster on this novel carbon material. After 3 days in culture, there is a precocious increase in the frequency of neuronal activity and in the expression of maturation marker KCC2 on carbon films as compared to a commonly used glass surface. Accelerated development is accompanied by a dramatic increase in neuronal dendrite arborization. The mechanism for the precocious maturation involves the act

SUBMITTER: Ludwig A 

PROVIDER: S-EPMC7573613 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature