Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids.

ABSTRACT: Three-dimensional (3D) cell culture technologies, such as organoids, are physiologically relevant models for basic and clinical applications. Automated microfluidics offers advantages in high-throughput and precision analysis of cells but is not yet compatible with organoids. Here, we present an automated, high-throughput, microfluidic 3D organoid culture and analysis system to facilitate preclinical research and personalized therapies. Our system provides combinatorial and dynamic drug treatments to hundreds of cultures and enables real-time analysis of organoids. We validate our system by performing individual, combinatorial, and sequential drug screens on human-derived pancreatic tumor organoids. We observe significant differences in the response of individual patient-based organoids to

SUBMITTER: Schuster B 

PROVIDER: S-EPMC7573629 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature