Baicalin Inhibits Influenza A Virus Infection via Promotion of M1 Macrophage Polarization.

ABSTRACT:

Background and aims

The natural compound baicalin (BA) possesses potent antiviral properties against the influenza virus. However, the underlying molecular mechanisms of this antiviral activity and whether macrophages are involved remain unclear. In this study, we, therefore, investigated the effect of BA on macrophages.

Methods

We studied macrophage recruitment, functional phenotypes (M1/M2), and the cellular metabolism via flow cytometry, qRT-PCR, immunofluorescence, a cell culture transwell system, and GC-MS-based metabolomics both in vivo in H1N1 A virus-infected mice and in vitro.

Results

BA treatment drastically reduced macrophage recruitment (CD11b⁺, F4/80⁺) by approximately 90% while maintaining the proportion of M1-p

SUBMITTER: Geng P 

PROVIDER: S-EPMC7574031 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature