High throughput single-cell detection of multiplex CRISPR-edited gene modifications.

ABSTRACT: CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.

SUBMITTER: Ten Hacken E 

PROVIDER: S-EPMC7574538 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature