GLT-1 Knockdown Inhibits Ceftriaxone-Mediated Improvements on Cognitive Deficits, and GLT-1 and xCT Expression and Activity in APP/PS1 AD Mice.

ABSTRACT:

Objective

Glutamate transporter-1 (GLT-1) and system x _c ^- mediate glutamate uptake and release, respectively. Ceftriaxone has been reported to upregulate GLT-1 expression and improve cognitive decline in APP/PS1 mice. The aim of the present study was to elucidate the role of GLT-1 in ceftriaxone-mediated improvement on cognitive deficits and associated changes in xCT (catalytic subunit of system x _c ^-) expression and activity using GLT-1 knockdown APP/PS1 mice.

Methods

GLT-1 knockdown (GLT-1^±) mice were generated in C57BL/6J mice using the CRISPR/Cas9 technique and crossed to APP/PS1 mice to generate GLT-1^±APP/PS1 mice. The cognition was evaluated by novel object recognition and Morris water maz

SUBMITTER: Gao J 

PROVIDER: S-EPMC7574737 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature