Large-Scale Topological Changes Restrain Malignant Progression in Colorectal Cancer.

ABSTRACT: Widespread changes to DNA methylation and chromatin are well documented in cancer, but the fate of higher-order chromosomal structure remains obscure. Here we integrated topological maps for colon tumors and normal colons with epigenetic, transcriptional, and imaging data to characterize alterations to chromatin loops, topologically associated domains, and large-scale compartments. We found that spatial partitioning of the open and closed genome compartments is profoundly compromised in tumors. This reorganization is accompanied by compartment-specific hypomethylation and chromatin changes. Additionally, we identify a compartment at the interface between the canonical A and B compartments that is reorganized in tumors. Remarkably, similar shifts were evident in non-malignant cells that hav

SUBMITTER: Johnstone SE 

PROVIDER: S-EPMC7575124 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature