Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research.

ABSTRACT: The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has received less attention. This study firstly evaluated the use of commercially available rodent diets across research institutions, with 28 different diets reported by 45 survey respondents. Secondly, highly variable ingredient, FODMAP (Fermentable Oligo-, Di-, Mono-saccharides And Polyols) and gluten content was found between different commercially available rodent diets. Finally, 40 mice were randomized to four groups, each receiving a different commercially available rodent diet, and the dieta

SUBMITTER: Tuck CJ 

PROVIDER: S-EPMC7575541 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature