Dataset Information

The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity.

ABSTRACT:

Background

The cellular immunity of lung cancer patients is mainly the immune response of T cells, which plays an important role in tumour cell killing and immune surveillance. Transforming growth factor 1 (TGF-β1) is secreted by tumour cells that can suppress the immune response and is an important group of immune down-regulation factors. Our study aims to investigate the effect of TGF-β1 on the morphology and cellular immune function of A549 and peripheral blood mononuclear cells (PBMCs).

Methods

A549 cell line was cultured, PBMCs were cultured with different concentrations of TGF-β1, and the morphology of A549 cells and PBMCs were seen. The levels of interleukin (IL)-2, IL-4, IL-6, IL-10, IFN-γ, and TNF and the numbers of CD3, CD4, CD8, CD4/CD8, and CD3 CD25 and CD4 CD25 in PBMCs were detected.

Results

During co-culture of A549 with PBMCs, TGF-β1 can induced A549 showing epithelial-to-mesenchymal transition, enhanced its ability of migration and infiltration. Simultaneously, TGF-β1 can depressing the growth and proliferation of PBMCs, inhibiting T-cell activation, and accelerating the PBMCs apoptosis. TGF-β1 can inhibits A549 Th1 related-cytokines, enhance Th2 related-cytokines, cause the disorder of Th1/Th2, resulting in the Th1 cellular dominate immunity decline.

Conclusions

TGF-β1 may affect the secretion of related cytokines, hinder the activation of T lymphocytes, destroy the immune surveillance and killing effect of the body, and thus inhibit the cellular immunity.

SUBMITTER: Li MJ

PROVIDER: S-EPMC7575940 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Publications

The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity.

Li Min-Jie MJ Wang Xi X Chen Ying Y Li Guang-Jian GJ Zhao Guang-Qiang GQ Xiang Bing-Quan BQ Wei Xue-Qiang XQ Lei Yu-Jie YJ Huang Yun-Chao YC

Annals of translational medicine 20200901 17

<h4>Background</h4>The cellular immunity of lung cancer patients is mainly the immune response of T cells, which plays an important role in tumour cell killing and immune surveillance. Transforming growth factor 1 (TGF-β1) is secreted by tumour cells that can suppress the immune response and is an important group of immune down-regulation factors. Our study aims to investigate the effect of TGF-β1 on the morphology and cellular immune function of A549 and peripheral blood mononuclear cells (PBMC ...[more]

PMID: 33145295

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Dataset Information

The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity.

Background

Methods

Results

Conclusions

Publications

The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity.

Similar Datasets

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