Expressions and relationship of Kruppel-like factor 15 and endothelial nitric oxide synthase in experimental deep venous thrombosis.

ABSTRACT:

Background

Deep vein thrombosis (DVT) is an early postoperative complication. Thrombosis formation, which is potentially life-threatening, seriously affects the rehabilitation of patients after surgery. We aimed to establish a C57 mouse model of DVT and to examine the changes in the expression of Krüppel-like factor 15 (KLF15) and endothelial nitric oxide synthase (eNOS) in venous wall tissues, and we also investigated the regulatory relationship of KLF15 and eNOS in the thrombin-induced human umbilical vein endothelial cell (HUVEC) injury cell model.

Methods

The DVT model was established using the inferior vena cava (IVC) stenosis method. The expression levels of KLF15 and eNOS were analyzed using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In ce

SUBMITTER: Yang X 

PROVIDER: S-EPMC7575959 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature