Schisandrin B synergizes docetaxel-induced restriction of growth and invasion of cervical cancer cells in vitro and in vivo.

ABSTRACT:

Background

Cervical cancer is a prevalent tumor in women. Here we investigated the synergic effects of Schisandrin B (Sch B), an active compound extracted from the Chinese herb Schisandra Chinensis, in docetaxel (DTX)-induced restriction of growth and invasion of cervical cancer.

Methods

Caski cells were treated with Sch B and DTX for 24 hours. In vitro effects were investigated with Cell counting kit-8, western blotting, colony-forming, Transwell, Annexin V-FITC enabled flow cytometry. Then, in vivo experiments were engaged with Sch B (20 mg/kg) and DTX (10 mg/kg) for 30 days, and IHC were applied to validate the effects in vivo.

Results

Both Sch B and DTX reduced cell viability, inhibited colony formatting, induced apoptosis, and limited

SUBMITTER: Yan C 

PROVIDER: S-EPMC7576040 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature