An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues.

ABSTRACT: Time-lapse imaging is an essential tool to study dynamic biological processes that cannot be discerned from fixed samples alone. However, imaging cell- and tissue-level processes in intact animals poses numerous challenges if the organism is opaque and/or motile. Explant cultures of intact tissues circumvent some of these challenges, but sample drift remains a considerable obstacle. We employed a simple yet effective technique to immobilize tissues in medium-bathed agarose. We applied this technique to study multiple Drosophila tissues from first-instar larvae to adult stages in various orientations and with no evidence of anisotropic pressure or stress damage. Using this method, we were able to image fine features for up to 18 h and make novel observations. Specifically, we report

SUBMITTER: Bostock MP 

PROVIDER: S-EPMC7576353 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature