Epigenetic Changes in Islets of Langerhans Preceding the Onset of Diabetes.

ABSTRACT: The identification of individuals with a high risk of developing type 2 diabetes (T2D) is fundamental for prevention. Here, we used a translational approach and prediction criteria to identify changes in DNA methylation visible before the development of T2D. Islets of Langerhans were isolated from genetically identical 10-week-old female New Zealand Obese mice, which differ in their degree of hyperglycemia and in liver fat content. The application of a semiexplorative approach identified 497 differentially expressed and methylated genes (P = 6.42e-09, hypergeometric test) enriched in pathways linked to insulin secretion and extracellular matrix-receptor interaction. The comparison of mouse data with DNA methylation levels of incident T2D cases from the prospective European Prospecti

SUBMITTER: Ouni M 

PROVIDER: S-EPMC7576562 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature