An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1.

ABSTRACT:

Background

Inhibition of DNA-binding of proteins by small-molecule chemicals holds immense potential in manipulating the activities of DNA-binding proteins. Such a chemical inhibition of DNA-binding of proteins can be used to modulate processes such as replication, transcription, DNA repair and maintenance of epigenetic states. This prospect is currently challenged with the absence of robust and generic protocols to identify DNA-protein interactions. Additionally, much of the current approaches to designing inhibitors requires structural information of the target proteins.

Methods

We have developed a simple dot blot and immunodetection-based assay to screen chemical libraries for inhibitors of DNA-protein interactions. The assay has been applied to a library of 1685 FDA-appr

SUBMITTER: Patel M 

PROVIDER: S-EPMC7576722 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature