SNP barcodes provide higher resolution than microsatellite markers to measure Plasmodium vivax population genetics.

ABSTRACT:

Background

Genomic surveillance of malaria parasite populations has the potential to inform control strategies and to monitor the impact of interventions. Barcodes comprising large numbers of single nucleotide polymorphism (SNP) markers are accurate and efficient genotyping tools, however may need to be tailored to specific malaria transmission settings, since 'universal' barcodes can lack resolution at the local scale. A SNP barcode was developed that captures the diversity and structure of Plasmodium vivax populations of Papua New Guinea (PNG) for research and surveillance.

Methods

Using 20 high-quality P. vivax genome sequences from PNG, a total of 178 evenly spaced neutral SNPs were selected for development of an amplicon sequencing assay combining a series of multiplex

SUBMITTER: Fola AA 

PROVIDER: S-EPMC7576724 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature