Time course of altered DNA methylation evoked by critical illness and by early administration of parenteral nutrition in the paediatric ICU.

ABSTRACT:

Background

A genome-wide study identified de novo DNA methylation alterations in leukocytes of children at paediatric intensive care unit (PICU) discharge, offering a biological basis for their impaired long-term development. Early parenteral nutrition (early-PN) in PICU, compared with omitting PN in the first week (late-PN), explained differential methylation of 23% of the affected CpG-sites. We documented the time course of altered DNA methylation in PICU and the impact hereon of early nutritional management.

Results

We selected 36 early-PN and 36 late-PN matched patients, and 42 matched healthy children. We quantified DNA methylation on days 3, 5 and 7 for the 147 CpG-sites of which methylation was normal upon PICU admission in this subset and altered by critical illness

SUBMITTER: Verlinden I 

PROVIDER: S-EPMC7576729 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature