Detection and discrimination of multiple strains of Zika virus by reverse transcription-loop-mediated isothermal amplification.

ABSTRACT:

Background

Monitoring both invasion of Zika virus disease into free countries and circulation in endemic countries is essential to avoid a global pandemic. However, the difficulty lies in detecting Zika virus due to the large variety of mutations in its genomic sequence. To develop a rapid and simple method with high accuracy, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was adopted for the detection of Zika virus strains derived from several countries.

Results

Common primers for RT-LAMP were designed based on the genomic sequences of two standard Zika strains: African lineage, MR-766, and Asian lineage, PRVABC59. RT-LAMP reactions using a screened primer set, targeting the NS3 region, detected both Zika virus strains. The minimum detectable quantit

SUBMITTER: Aonuma H 

PROVIDER: S-EPMC7576873 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature