Collapse of Global Neuronal States in Caenorhabditis elegans under Isoflurane Anesthesia.

ABSTRACT:

Background

A comprehensive understanding of how anesthetics facilitate a reversible collapse of system-wide neuronal function requires measurement of neuronal activity with single-cell resolution. Multineuron recording was performed in Caenorhabditis elegans to measure neuronal activity at varying depths of anesthesia. The authors hypothesized that anesthesia is characterized by dyssynchrony between neurons resulting in a collapse of organized system states.

Methods

Using light-sheet microscopy and transgenic expression of the calcium-sensitive fluorophore GCaMP6s, a majority of neurons (n = 120) in the C. elegans head were simultaneously imaged in vivo and neuronal activity was measured. Neural activity and system-wide dynamics were compared in 10 animals, progressively dos

SUBMITTER: Awal MR 

PROVIDER: S-EPMC7577177 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature