Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse.

ABSTRACT: We generated an NOD/Shi-scid-IL2Rγ ^null (NOG) mouse deficient for the Fcer1g and Fcgr2b genes (NOG-FcγR^-/- mice), in which monocytes/macrophages do not express activating (FcγRI, III, and IV) or inhibitory (FcγRIIB) Fcγ receptors. Antibody-dependent cellular cytotoxicity (ADCC) by innate immune cells was strongly reduced in this strain. Thus, while the growth of xenogeneic human tumors engrafted in conventional NOG mice was suppressed by innate cells upon specific antibody treatment, such growth inhibition was abrogated in NOG-FcγR^-/- mice. Using this novel strain, we further produced NOG-FcγR^-/--mice expressing human IL-15 (NOG-FcγR^-/--hIL-15 Tg). The mice inherited unique features from each strain, i.e., t

SUBMITTER: Katano I 

PROVIDER: S-EPMC7577188 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature