MCART1/SLC25A51 is required for mitochondrial NAD transport.

ABSTRACT: The nicotinamide adenine dinucleotide (NAD⁺/NADH) pair is a cofactor in redox reactions and is particularly critical in mitochondria as it connects substrate oxidation by the tricarboxylic acid (TCA) cycle to adenosine triphosphate generation by the electron transport chain (ETC) and oxidative phosphorylation. While a mitochondrial NAD⁺ transporter has been identified in yeast, how NAD enters mitochondria in metazoans is unknown. Here, we mine gene essentiality data from human cell lines to identify MCART1 (SLC25A51) as coessential with ETC components. MCART1-null cells have large decreases in TCA cycle flux, mitochondrial respiration, ETC complex I activity, and mitochondrial levels of NAD⁺ and NADH. Isolated mitochondria from cells la

SUBMITTER: Kory N 

PROVIDER: S-EPMC7577609 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature