Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon-anticodon pairing.

ABSTRACT: Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguanosine modification at guanine nucleotide 37 (m¹G37) located in the anticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36. The absence of m¹G37 in tRNA^Pro causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNA^Pro bound to either cognate or slippery codons to determine how the m¹G37 modification prevents mRNA frameshifting. The structures reveal that certain codon-anticodon contexts and the lack of m¹G37

SUBMITTER: Hoffer ED 

PROVIDER: S-EPMC7577736 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature