Cell-Cycle-Associated Expression Patterns Predict Gene Function in Mycobacteria.

ABSTRACT: Although the major events in prokaryotic cell cycle progression are likely to be coordinated with transcriptional and metabolic changes, these processes remain poorly characterized. Unlike many rapidly growing bacteria, DNA replication and cell division are temporally resolved in mycobacteria, making these slow-growing organisms a potentially useful system to investigate the prokaryotic cell cycle. To determine whether cell-cycle-dependent gene regulation occurs in mycobacteria, we characterized the temporal changes in the transcriptome of synchronously replicating populations of Mycobacterium tuberculosis (Mtb). By enriching for genes that display a sinusoidal expression pattern, we discover 485 genes that oscillate with a period consistent with the cell cycle. During cytokinesis, the tim

SUBMITTER: Bandekar AC 

PROVIDER: S-EPMC7578119 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature