Cell Migration Driven by Self-Generated Integrin Ligand Gradient on Ligand-Labile Surfaces.

ABSTRACT: Integrin-ligand interaction mediates the adhesion and migration of many metazoan cells. Here, we report a unique mode of cell migration elicited by the lability of integrin ligands. We found that stationary cells spontaneously turn migratory on substrates where integrin ligands are subject to depletion by cellular force. Using TGT, a rupturable molecular linker, we quantitatively tuned the rate of ligand rupture by cellular force and tested platelets (anucleate cells), CHO-K1 cells (nucleated cells), and other cell types on TGT surfaces. These originally stationary cells readily turn motile on the uniform TGT surface, and their motility is correlated with the ligand depletion rate caused by cells. We named this new migration mode ligand-depleting (LD) migration. Through both experiments an

SUBMITTER: Sarkar A 

PROVIDER: S-EPMC7578120 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature