A six-plex droplet digital RT-PCR assay for seasonal influenza virus typing, subtyping, and lineage determination.

ABSTRACT:

Background

There are two influenza A subtypes (H1 and H3) and two influenza B lineages (Victoria and Yamagata) that currently co-circulate in humans. In this study, we report the development of a six-plex droplet digital RT-PCR (ddRT-PCR) assay that can detect HA and M segments of influenza A (H1, H3, and M) and influenza B (Yamagata HA, Victoria HA, and M) viruses in a single reaction mixture. It can simultaneously detect six different nucleic acid targets in a ddRT-PCR platform.

Methods

The six-plex ddRT-PCR used in this study is an amplitude-based multiplex assay. The analytical performance of the assay was evaluated. Correlation with standard qRT-PCR methodology was assessed using 55 clinical samples.

Results

The assay has a wide dynamic range, and it has good rep

SUBMITTER: Leong NKC 

PROVIDER: S-EPMC7578307 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature