Comparison of the next-generation sequencing (NGS) technology with culture methods in the diagnosis of bacterial and fungal infections.

ABSTRACT:

Background

Bacterial and fungal infections that caused by various kinds of pathogens are frequently-occurring disease all over the world. To overcome the shortcomings of traditional culture method, we have adapted next-generation sequencing (NGS) technology to identify pathogens.

Methods

In this study, clinical samples from 20 patients pre-diagnosed with bacterial and fungal infections in the Shanghai Pulmonary Hospital of Tongji University, China, were investigated retrospectively to compare the NGS with conventional "gold standard" culture methods.

Results

The detection rates of bacterial or fungal infections were 95.0% (19/20) by NGS and 60.0% (12/20) by culture method. There was a significant difference between the results of NGS and traditional culture method by

SUBMITTER: Chen P 

PROVIDER: S-EPMC7578456 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature