Newly established gastrointestinal cancer cell lines retain the genomic and immunophenotypic landscape of their parental cancers.

ABSTRACT: Human cancer cell lines are frequently used as model systems to study molecular mechanisms and genetic changes in cancer. However, the model is repeatedly criticized for its lack of proximity to original patient tumors. Therefore, understanding to what extent cell lines cultured under artificial conditions reflect the phenotypic and genomic profiles of their corresponding parental tumors is crucial when analyzing their biological properties. To directly compare molecular alterations between patient tumors and derived cell lines, we have established new cancer cell lines from four patients with gastrointestinal tumors. Tumor entities comprised esophageal cancer, colon cancer, rectal cancer and pancreatic cancer. Phenotype and genotype of both patient tumors and derived low-passage cell line

SUBMITTER: Hirsch D 

PROVIDER: S-EPMC7578805 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature