Single-cell lineage tracing unveils a role for TCF15 in haematopoiesis.

ABSTRACT: Bone marrow transplantation therapy relies on the life-long regenerative capacity of haematopoietic stem cells (HSCs)^1,2. HSCs present a complex variety of regenerative behaviours at the clonal level, but the mechanisms underlying this diversity are still undetermined^3-11. Recent advances in single-cell RNA sequencing have revealed transcriptional differences among HSCs, providing a possible explanation for their functional heterogeneity^12-17. However, the destructive nature of sequencing assays prevents simultaneous observation of stem cell state and function. To solve this challenge, we implemented expressible lentiviral barcoding, which enabled simultaneous analysis of lineages and transcriptomes from single adult HSCs and their clonal trajectories durin

SUBMITTER: Rodriguez-Fraticelli AE 

PROVIDER: S-EPMC7579674 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature