Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells.

ABSTRACT: Mesenchymal stromal cells are promising candidates for regenerative applications upon treatment of bone defects. Bone marrow-derived stromal cells (BMSCs) are limited by yield and donor morbidity but show superior osteogenic capacity compared to adipose-derived stromal cells (ASCs), which are highly abundant and easy to harvest. The underlying reasons for this difference on a proteomic level have not been studied yet. Human ASCs and BMSCs were characterized by FACS analysis and tri-lineage differentiation, followed by an intraindividual comparative proteomic analysis upon osteogenic differentiation. Results of the proteomic analysis were followed by functional pathway analysis. 29 patients were included with a total of 58 specimen analysed. In these, out of 5148 identified proteins 2095 co

SUBMITTER: Dadras M 

PROVIDER: S-EPMC7579700 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature