De novo transcriptome assembly from the gonads of a scleractinian coral, Euphyllia ancora: molecular mechanisms underlying scleractinian gametogenesis.

ABSTRACT:

Background

Sexual reproduction of scleractinians has captured the attention of researchers and the general public for decades. Although extensive ecological data has been acquired, underlying molecular and cellular mechanisms remain largely unknown. In this study, to better understand mechanisms underlying gametogenesis, we isolated ovaries and testes at different developmental phases from a gonochoric coral, Euphyllia ancora, and adopted a transcriptomic approach to reveal sex- and phase-specific gene expression profiles. In particular, we explored genes associated with oocyte development and maturation, spermiogenesis, sperm motility / capacitation, and fertilization.

Results

1.6 billion raw reads were obtained from 24 gonadal samples. De novo assembly of trimmed reads, an

SUBMITTER: Chiu YL 

PROVIDER: S-EPMC7579821 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature