Aging-relevant human basal forebrain cholinergic neurons as a cell model for Alzheimer's disease.

ABSTRACT:

Background

Alzheimer's disease (AD) is an adult-onset mental disorder with aging as a major risk factor. Early and progressive degeneration of basal forebrain cholinergic neurons (BFCNs) contributes substantially to cognitive impairments of AD. An aging-relevant cell model of BFCNs will critically help understand AD and identify potential therapeutics. Recent studies demonstrate that induced neurons directly reprogrammed from adult human skin fibroblasts retain aging-associated features. However, human induced BFCNs (hiBFCNs) have yet to be achieved.

Methods

We examined a reprogramming procedure for the generation of aging-relevant hiBFCNs through virus-mediated expression of fate-determining transcription factors. Skin fibroblasts were obtained from healthy young persons, h

SUBMITTER: Ma S 

PROVIDER: S-EPMC7579825 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature