Hypoxia directed migration of human naive monocytes is associated with an attenuation of cytokine release: indications for a key role of CCL26.

ABSTRACT:

Background

Numerous tissue-derived factors have been postulated to be involved in tissue migration of circulating monocytes. The aim of this study was to evaluate whether a defined hypoxic gradient can induce directed migration of naïve human monocytes and to identify responsible autocrine/paracrine factors.

Methods

Monocytes were isolated from peripheral blood mononuclear cells, transferred into chemotaxis chambers and subjected to a defined oxygen gradient with or without the addition of CCL26. Cell migration was recorded and secretome analyses were performed.

Results

Cell migration recordings revealed directed migration of monocytes towards the source of hypoxia. Analysis of the monocyte secretome demonstrated a reduced secretion of 70% (19/27) of the analyzed cyto

SUBMITTER: Hummitzsch L 

PROVIDER: S-EPMC7579884 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature