Metabolic plasticity of IDH1-mutant glioma cell lines is responsible for low sensitivity to glutaminase inhibition.

ABSTRACT:

Background

Targeting glutamine metabolism in cancer has become an increasingly vibrant area of research. Mutant IDH1 (IDH1 ^mut ) gliomas are considered good candidates for targeting this pathway because of the contribution of glutamine to their newly acquired function: synthesis of 2-hydroxyglutarate (2HG).

Methods

We have employed a combination of ¹³C tracers including glutamine and glucose for investigating the metabolism of patient-derived IDH1 ^mut glioma cell lines through NMR and LC/MS. Additionally, genetic loss-of-function (in vitro and in vivo) approaches were performed to unravel the adaptability of these cell lines to the inhibition of glutaminase activity.

Results

We report the adaptability of IDH1 ^mut<

SUBMITTER: Ruiz-Rodado V 

PROVIDER: S-EPMC7579920 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature