Protocol for Genome Editing to Produce Multiple Mutants in Wheat.
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ABSTRACT:
SUBMITTER: Abe F
PROVIDER: S-EPMC7580089 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
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Protocol for Genome Editing to Produce Multiple Mutants in Wheat.
Abe Fumitaka F Ishida Yuji Y Hisano Hiroshi H Endo Masaki M Komari Toshihiko T Toki Seiichi S Sato Kazuhiro K
STAR protocols 20200613 2
Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (<i>Triticum aestivum</i>) cv. Fielder. Using <i>Agrobacterium</i>-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for mutant production. The mutants produced by this protocol can be used ...[more]
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