Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes.

ABSTRACT: The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018.

SUBMITTER: Kleinsorge M 

PROVIDER: S-EPMC7580117 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature