Transgenic Expression of PRSS1^R122H Sensitizes Mice to Pancreatitis.

ABSTRACT:

Background & aims

Mutations in the trypsinogen gene (PRSS1) cause human hereditary pancreatitis. However, it is not clear how mutant forms of PRSS1 contribute to disease development. We studied the effects of expressing mutant forms of human PRSS1 in mice.

Methods

We expressed forms of PRSS1 with and without the mutation encoding R122H (PRSS1^R122H) specifically in pancreatic acinar cells under control of a full-length pancreatic elastase gene promoter. Mice that did not express these transgenes were used as controls. Mice were given injections of caerulein to induce acute pancreatitis or injections of lipopolysaccharide to induce chronic pancreatitis. Other groups of mice were fed ethanol or placed on a high-fat diet to induce pancreatitis. Pancreata were collecte

SUBMITTER: Huang H 

PROVIDER: S-EPMC7580257 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature