MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.

ABSTRACT: N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination. By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible. Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics). We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M - H]^- species of N

SUBMITTER: Heijs B 

PROVIDER: S-EPMC7581013 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature