Automated Annotation of Sphingolipids Including Accurate Identification of Hydroxylation Sites Using MSⁿ Data.

ABSTRACT: Sphingolipids constitute a heterogeneous lipid category that is involved in many key cellular functions. For high-throughput analyses of sphingolipids, tandem mass spectrometry (MS/MS) is the method of choice, offering sufficient sensitivity, structural information, and quantitative precision for detecting hundreds to thousands of species simultaneously. While glycerolipids and phospholipids are predominantly non-hydroxylated, sphingolipids are typically dihydroxylated. However, species containing one or three hydroxylation sites can be detected frequently. This variability in the number of hydroxylation sites on the sphingolipid long-chain base and the fatty acyl moiety produces many more isobaric species and fragments than for other lipid categories. Due to this complexity, the automated

SUBMITTER: Hartler J 

PROVIDER: S-EPMC7581017 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature