Combination product of dermal matrix, human mesenchymal stem cells, and timolol promotes diabetic wound healing in mice.

ABSTRACT: Diabetic foot ulcers are a major health care concern with limited effective therapies. Mesenchymal stem cell (MSC)-based therapies are promising treatment options due to their beneficial effects of immunomodulation, angiogenesis, and other paracrine effects. We investigated whether a bioengineered scaffold device containing hypoxia-preconditioned, allogeneic human MSCs combined with the beta-adrenergic antagonist timolol could improve impaired wound healing in diabetic mice. Different iterations were tested to optimize the primary wound outcome, which was percent of wound epithelialization. MSC preconditioned in 1 μM timolol at 1% oxygen (hypoxia) seeded at a density of 2.5 × 10⁵  cells/cm² on Integra Matrix Wound Scaffold (MSC/T/H/S) applied to wounds and combined wi

SUBMITTER: Yang HY 

PROVIDER: S-EPMC7581456 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature